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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
基质金属蛋白酶7(MMP-7),也称为基质溶解素(MAT),pump-1蛋白酶或子宫金属蛋白酶,是由MMP7基因编码的酶。该酶也被称为苦参碱。 活化的MMP7的主要作用是通过降解大分子(包括酪蛋白,I型,II型,IV型和V型明胶,纤连蛋白和蛋白聚糖)来分解细胞外基质。它的cDNA与基质溶素-1同源49%。与MMP家族的其他成员相比,它没有C端蛋白结构域。与具有最长铰链的MMP9相比,它缺乏血红素并且没有铰链。相反,它含有可变的C端血红素样结构域,有助于底物特异性它能作用于明胶和FN。它的表达由Wnt/β连环蛋白信号通路调控,并由转化生长因子β(TGF-β介导)。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠基质金属蛋白酶7(MMP7)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠基质金属蛋白酶7(MMP7)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MMP7. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MMP7 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.