计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
基质金属蛋白酶8(MMP-8),又名中性粒细胞胶原酶或PMNL胶原酶,是一种存在于大多数哺乳动物结缔组织中的胶原裂解酶,由MMP8基因编码,该基因是一簇MMP基因的一部分,这些基因定位于染色体11q22.3。多种物质,如IL-1、IL-8、TNF-α和GM-CSF,可刺激中性粒细胞释放MMP-8,后者是启动ECM分解的关键酶。它的主要功能是I型,II型和III型胶原蛋白的降解。活化的它可裂解天然蛋白如纤连蛋白、软骨蛋白聚糖、丝氨酸蛋白酶抑制蛋白和多肽。它通过预防淋巴结转移来影响细胞的转移行为,强调药物开发中抗目标识别的重要性。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠基质金属蛋白酶8(MMP-8)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠基质金属蛋白酶8(MMP-8)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MMP-8. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MMP-8 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.