BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
墨蝶呤还原酶（SPR）是一种重要的生物酶，由SPR基因编码，属于短链脱氢酶/还原酶家族。它在四氢生物蝶呤（BH4）的生物合成中起关键作用，而BH4是多种激素、炎症因子和神经递质合成的必需辅因子。SPR广泛分布于各组织中，与脑功能障碍、慢性疼痛、心血管疾病和癌症等多种疾病相关。在镇痛领域，SPR是一个有价值的靶点，已有多种SPR抑制剂药物被研发出来。此外，SPR基因突变可能导致墨蝶呤还原酶缺乏，进而影响多巴胺的合成和代谢，引发多巴反应性肌张力障碍等疾病。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠墨蝶呤还原酶(SPR)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠墨蝶呤还原酶(SPR)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SPR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SPR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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