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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尼克酰胺-N-甲基转移酶,也被称为 NNMT,是由 NNMT 基因编码的酶。它使用甲基供体 S-腺苷甲硫氨酸(SAM-e)催化烟酰胺和类似化合物的甲基化,以产生 S-腺苷-L-同型半胱氨酸(SAH)和 1-甲基烟酰胺。它和 SAM-e 对烟酰胺的甲基化是烟酰胺降解导致尿液排泄的主要途径。它在人肝脏中高度表达。它的人类胚胎干细胞表达被认为有助于将细胞维持在幼稚状态。NNMT 表达在许多癌症中显着上调,包括胰腺癌,其中 NNMT 酶水平与死亡风险增加相关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠尼克酰胺-N-甲基转移酶(NNMT)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠尼克酰胺-N-甲基转移酶(NNMT)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse NNMT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse NNMT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.