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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Nestin 是一种蛋白质,是由 NES 基因编码的。Nestin(巢蛋白)是一种 VI型中间丝(IF)蛋白。这些中间丝蛋白主要在神经细胞中表达,它们与轴突的径向生长有关系。七个基因编码重神经丝(NF-H)、中神经丝(NF-M)和轻神经丝(NF-L)蛋白,神经细胞中的 nestin 和α-internexin,肌肉细胞中的 synemin α和 desmuslin/synemin β(DMN 基因的两个备选转录本),以及 syncoilin(也在肌肉细胞中)。这一组的成员大多喜欢在组织中以异质聚合物的形式联合起来。nestin 在体外形成同源二聚体和同源四聚体,但本身并不形成 IF。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠巢蛋白(Nestin)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠巢蛋白(Nestin)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Nestin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Nestin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.