BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
巨噬细胞集落刺激因子(M-CSF)也称为集落刺激因子-1(CSF-1),是一种具有谱系特异性的细胞因子。M-CSF为链间二硫键连接而成的二聚体糖蛋白，对单核细胞的增殖、分化及维持其活性有重要作用。其受体为c-Fms。M-CSF可在间质细胞如成纤维细胞、成骨细胞及内皮细胞中合成,也可在激活的巨噬细胞、B细胞、T细胞及多种肿瘤细胞中产生。M-CSF诱导破骨细胞发生破骨细胞，为单核-巨噬细胞系成员,是由造血系统的单核、巨噬细胞的前体细胞融合而成。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠巨噬细胞集落刺激因子(M-CSF)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠巨噬细胞集落刺激因子(M-CSF)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse M-CSF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse M-CSF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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