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原癌基因c-KIT是编码受体酪氨酸激酶蛋白的基因,被称为酪氨酸蛋白激酶KIT、CD117(分化簇117)或肥大/干细胞生长因子受体(SCFR)。已发现该基因有多种转录变体,编码不同的异构体。KIT最早由德国生物化学家Axel Ullrich于1987年描述为猫科动物肉瘤病毒癌基因v-kit的细胞同源物。KIT是一种细胞因子受体,在造血干细胞以及其他细胞类型表面表达。该受体的改变形式可能与某些类型的癌症有关。KIT是一种受体酪氨酸激酶III型,它与干细胞因子(一种能使某些类型的细胞生长的物质)结合,也被称为 "钢因子 "或 "c-kit配体"。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠干细胞因子受体(SCFR;CD117)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠干细胞因子受体(SCFR;CD117)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SCFR;CD117. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SCFR;CD117 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.