计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
异柠檬酸脱氢酶 1(IDH1),也被称为 NADP+,是一种由 2 号染色体上的 IDH1 基因编码的酶。异柠檬酸脱氢酶催化异柠檬酸氧化脱羧为 2-氧代戊二 酸。这些酶属于两种截然不同的亚类,其中一个使用 NAD+ 作为电子受体, 另一个使用 NADP+。已报道了五种异柠檬酸脱氢酶:三种 NAD+依赖性异 柠檬酸脱氢酶,定位于线粒体基质,以及两种 NADP+依赖性异柠檬酸脱氢 酶,其中一种是线粒体,另一种主要是细胞溶质。每个 NADP+依赖性同工 酶都是同型二聚体。该基因编码的蛋白质是在细胞质和过氧化物酶体中发现 的 NADP+依赖性异柠檬酸脱氢酶。它包含PTS-1过氧化物酶体靶向信号序 列。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠异柠檬酸脱氢酶1(IDH1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠异柠檬酸脱氢酶1(IDH1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse IDH1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse IDH1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.