计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
弹性蛋白是细胞外基质的一种关键蛋白质。它具有高度的弹性,存在于结缔组织中,使身体的许多组织在拉伸或收缩后恢复其形状。弹性蛋白帮助皮肤在被戳或捏的时候恢复到原来的位置。弹性蛋白也是脊椎动物身体中重要的承重组织,用于需要储存机械能的地方。在人类中,弹性蛋白是由ELN基因编码的。ELN基因编码的蛋白质是弹性纤维的两个组成部分之一。编码的蛋白质富含疏水性氨基酸,如甘氨酸和脯氨酸,它们形成由赖氨酸残基之间的交联所约束的移动疏水区域。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠弹性蛋白(Elastin)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠弹性蛋白(Elastin)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Elastin. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Elastin present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.