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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
成纤维细胞生长因子14是一种具有生物活性的蛋白质，是由FGF14基因编码的。该基因所编码的蛋白是成纤维细胞生长因子（FGF）家族的成员。FGF家族成员具有广泛的有丝分裂和细胞存活活性，参与各种生物过程，包括胚胎发育、细胞生长、形态发生、组织修复、肿瘤生长和侵袭。该基因的突变与常染色体显性脑共济失调有关。已发现该基因有替代性剪接的转录变体。FGF14主要在中枢神经系统中表达，与神经退行性疾病有关，如脊髓小脑共济失调（SAC27）。FGF14的缺乏也会损害海马区细胞的成熟，这可能与精神分裂症的发生有关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠成纤维细胞生长因子14(FGF14)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠成纤维细胞生长因子14(FGF14)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse FGF14. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse FGF14 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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