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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
无翅型MMTV整合位点家族成员10B(WNT10B),也被称为WNT12,是由WNT10B基因编码的蛋白质。WNT基因家族由结构相关的基因组成,这些基因编码分泌的信号转导蛋白。这些蛋白质与肿瘤发生和一些发育过程有关,包括胚胎发生过程中细胞命运和模式的调节。该基因是WNT基因家族的成员。它可能与乳腺癌有关,据推测,它的蛋白质信号传导是支配脂肪形成的分子开关。该基因与染色体12q13区域的另一个家庭成员WNT1聚集在一起。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠无翅型MMTV整合位点家族成员10B(WNT10B)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠无翅型MMTV整合位点家族成员10B(WNT10B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse WNT10B. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse WNT10B present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.