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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
末端补体复合物C5b-9(TCC)是一种通常在病原体细胞膜表面形成的蛋白质复合物,是宿主补体系统激活的结果,因此是免疫系统的一种效应物。抗体介导的补体激活导致TCC沉积在受感染的细胞表面。TCC的组装导致孔隙破坏目标细胞的细胞膜,导致细胞裂解和死亡。TCC是由补体成分C5b、C6、C7、C8和几个C9分子组成,也是由四个补体蛋白(C5b、C6、C7和C8)组成的复合物,它们与质膜的外表面结合,第五个蛋白(C9)的许多拷贝相互勾连,在膜上形成一个环。C9形成的环状结构是膜上的一个孔,允许分子自由扩散进入和离开细胞。如果形成足够多的孔隙,细胞就不再能够生存。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠末端补体复合物C5b-9(TCC C5b-9)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠末端补体复合物C5b-9(TCC C5b-9)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TCC C5b-9. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TCC C5b-9 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.