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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
极低密度脂蛋白亚型2(VLDL2)是极低密度脂蛋白(VLDL)的一种亚型,由肝脏合成并分泌到血液中。VLDL2富含甘油三酯,主要功能是将内源性甘油三酯运输至外周组织,如脂肪和肌肉细胞,供其储存或利用。在代谢过程中,VLDL2在脂蛋白脂肪酶(LPL)的作用下逐步释放甘油三酯,颗粒逐渐缩小,密度增加,最终转化为中间密度脂蛋白(IDL)或低密度脂蛋白(LDL)。VLDL2水平升高通常与甘油三酯代谢异常相关,可能增加心血管疾病的风险,如动脉粥样硬化。其代谢受饮食、胰岛素敏感性及激素水平等因素影响。临床上,VLDL2的检测常结合血脂谱分析,用于评估脂质代谢紊乱及心血管风险。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠极低密度脂蛋白亚型2(VLDL2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠极低密度脂蛋白亚型2(VLDL2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse VLDL2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse VLDL2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.