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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
海藻糖酶(trehalase),也被称为TREH,是由TREH基因编码的酶。它是糖苷水解酶,由小肠刷状缘中的细胞产生,催化海藻糖向葡萄糖的转化。它在大多数动物中被发现。非还原性双糖海藻糖(α-D-吡喃葡萄糖基-1,1-α-D-吡喃葡萄糖苷)是最重要的储存碳水化合物之一,几乎由除哺乳动物以外的所有生命形式产生。二糖通过酶海藻酶水解成两个葡萄糖分子。在酿酒酵母中发现了两种类型的海藻酶,即中性海藻酶(NT)和酸性海藻酶(AT),根据其pH值最优分类。NT的最佳pH值为7.0,而AT的最佳pH值为4.5。有报道称,酿酒酵母中超过90%的总AT活性是细胞外,并将细胞外海藻糖切割成质周空间中的葡萄糖。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠海藻糖酶(Trehalase)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠海藻糖酶(Trehalase)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Trehalase. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Trehalase present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.