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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
淋巴细胞激活基因 3,又称 LAG-3,是一种蛋白质,由 LAG3 基因编码。LAG3 于 1990 年被发现,在 2000 年被命名为 CD223(cluster of differentiation 223),是一种细胞表面分子,对 T 细胞功能有多种生物效应。它是一种免 疫检查点受体,因此是制药公司寻求开发癌症和自身免疫性疾病新疗法的各 种药物开发项目的目标。它的可溶性形式也正在被开发为一种癌症药物,其 本身也是如此。LAG3 基因包含 8 个外显子。序列数据、外显子/内含子组织 和染色体定位都表明 LAG3 与 CD4 关系密切。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠淋巴细胞活化基因3(LAG3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠淋巴细胞活化基因3(LAG3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse LAG3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse LAG3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.