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激活素受体样激酶1(ACVRL1),也被称为丝氨酸/苏氨酸蛋白激酶受体 R3 或 ALK1,是由 ACVRL1 基因编码的酶。它是 TGF beta 信号通路中的受体。 该基因编码 TGF-β配体超家族的 I 型细胞表面受体。它与其他 I 型受体在丝 氨酸-苏氨酸激酶亚结构域、激酶结构域之前的富含甘氨酸和丝氨酸的区域 (称为 GS 结构域)和 C 末端短尾方面具有高度相似性。编码的蛋白质与其 他密切相关的 ALK 或激活素受体样激酶蛋白具有相似的结构域结构,这些蛋 白形成受体丝氨酸/苏氨酸激酶的亚家族。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠激活素受体样激酶1(ACVRL1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠激活素受体样激酶1(ACVRL1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ACVRL1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ACVRL1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.