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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
玻连蛋白,也被称为 VTN 或 VN,是血红素家族的糖蛋白,在血清,细胞外基质和骨骼中大量存在,由 VTN 基因编码。它与整合素α-V β-3 结合,从而促进细胞粘附和扩散。它还抑制末端细胞溶解补体途径的膜损伤作用,并与几种 serpins(丝氨酸蛋白酶抑制剂)结合。它是一种分泌的蛋白质,以单链形式或由二硫键固定在一起的夹合的两链形式存在。它被推测参与止血和肿瘤恶性肿瘤。它是一种 54 kDa 糖蛋白,由 478 个氨基酸残基组成。蛋白质分子量的大约三分之一由碳水化合物组成。有时,蛋白质在精氨酸 379之后被切割,以产生双链玻连蛋白,其中两部分通过二硫键连接。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠玻连蛋白;体外粘连蛋白(VTN;CD51+CD61)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠玻连蛋白;体外粘连蛋白(VTN;CD51+CD61)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse VTN;CD51+CD61. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse VTN;CD51+CD61 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.