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甘露聚糖结合凝集素丝氨酸肽 1 又称甘露结合凝集素丝氨酸蛋白酶 1 和甘露糖相关丝氨酸蛋白酶 1（MASP-1），是一种由 MASP1 基因编码的酶。MASP-1 参与补体系统的凝集素途径，负责将 C4 和 C2 裂解成片段，形成C3 转化酶。MASP-1 是一种丝氨酸蛋白酶，作为补体激活的凝集素途径的一个组成部分发挥作用。补体途径在先天和适应性免疫反应中起着至关重要的作用。MASP-1以酶原形式合成，当它与凝集素途径的病原体识别分子-- 甘露糖结合凝集素和菲考林复合时被激活。该蛋白不直接参与补体激活，但可能通过裂解补体C2或激活另一种补体丝氨酸蛋白酶MASP-2而发挥补体激活的放大器的作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠甘露聚糖结合凝集素丝氨酸肽1(MASP1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠甘露聚糖结合凝集素丝氨酸肽1(MASP1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MASP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MASP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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