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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
癌基因产物(c-myc)是Myc家族家族中第一个被发现的基因,因为它与病毒基因v-myc同源。Myc是一个编码转录因子的调节器基因和原癌基因家族。 在癌症中,c-myc往往是组成型(持续)表达。这导致许多基因的表达增加,其中一些基因参与细胞增殖,有助于癌症的形成。涉及c-myc的常见易位对大多数伯基特淋巴瘤病例的发展至关重要。因此,它被视为抗癌药物的一个有希望的靶点。不幸的是,Myc具有几个特点,使其无法被治愈,因此任何针对Myc失调的抗癌药物都需要间接作用于该蛋白,即针对该蛋白的mRNA而不是针对该蛋白本身的小分子。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠癌基因产物(C-Myc)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠癌基因产物(C-Myc)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse C-Myc. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse C-Myc present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.