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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素1受体,II型(IL-1R2)也被称为CD121b(分化群121b)是一种白细胞介素受体。IL1R2也表示其基因。该基因所编码的蛋白是某些细胞因子的诱饵受体,属于白细胞介素-1受体家族。该蛋白与白细胞介素-1α(IL1A)、白细胞介素-1β(IL1B)和白细胞介素1受体拮抗剂(IL1Ra)结合,阻止它们与常规受体结合,从而抑制其信号的转导。IL-1R2蛋白还与IL-1受体信号的第二个组成部分,即IL-1RAcP,进行非生产性的相互作用,并且IL-1R2和IL-1RAcP胞外结构域与白细胞介素-1β的复合物已被X射线晶体学解决 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠白细胞介素1受体Ⅱ(IL-1R2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠白细胞介素1受体Ⅱ(IL-1R2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse IL-1R2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse IL-1R2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.