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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白介素 2 受体(IL-2R)是一种表达在某些免疫细胞(如淋巴细胞)表面的异构蛋白,它与一种叫做 IL-2 的细胞因子结合并作出反应。IL-2 与 IL-2 受体结合,IL-2 受体有三种形式,由三种不同的蛋白(通常称为 "链")的不同组合产生:α(α)(也称为 IL-2Rα、CD25 或 Tac 抗原)、β(β)(也称为 IL-2Rβ,或 CD122)和γ(γ)(也称为 IL-2Rγ、γc、普通γ链,或 CD132);这些亚单位也是其他细胞因子的受体的组成部分。IL-2R 的β和γ链是 I 型细胞因子受体家族的成员。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠白细胞介素2受体(IL-2R)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠白细胞介素2受体(IL-2R)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse IL-2R. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse IL-2R present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.