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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素7(IL-7)是一种由骨髓和胸腺中的基质细胞分泌的造血生长因子。它也由角质细胞、树突状细胞、肝细胞、神经元和上皮细胞产生, 但正常淋巴细胞不产生。 一项研究还证明了由T细胞急性淋巴细胞白血病(T-ALL)介导的IL-7细胞因子的自分泌如何参与T-ALL的致癌发展,并为T-ALL的传播提供新的见解。IL-7刺激多能造血干细胞分化为淋巴祖细胞(与髓系祖细胞相反,后者的分化是由IL-3刺激的)。 它还能刺激淋巴系所有细胞(B细胞、T细胞和NK细胞)的增殖。在B细胞成熟、T和NK细胞生存、发育和平衡的某些阶段,它对增殖非常重要。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠白细胞介素7(IL-7)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠白细胞介素7(IL-7)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse IL-7. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse IL-7 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.