计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Cortactin(来自 "皮质肌动蛋白结合蛋白")是一种位于细胞质中的单体蛋白,可被外部刺激激活,以促进肌动蛋白细胞骨架的聚合和重新排列,特别是细胞周边的肌动蛋白皮质,它存在于所有细胞类型中。当激活时,它将招募 Arp2/3 复合蛋白到现有的肌动蛋白微丝上,促进和稳定肌动蛋白分支的核点。Cortactin 对促进片状细胞的形成、入侵细胞的形成、细胞迁移和内吞作用很重要。与 CTTN 相关的疾病包括乙状窦腺癌和 Wiskott-Aldrich 综合征。它的相关途径包括发育缝隙-罗布信号传导和 Syndecan-3 介导的信号传导事件。与该基因相关的基因本体论注释包括 profilin 结合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠皮动蛋白(CTTN)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠皮动蛋白(CTTN)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse CTTN. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse CTTN present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.