计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
磷酸丙糖异构酶1(TPI1),也被称为TIM,是由TPI1基因编码的酶。该基因编码一种由两种相同蛋白质组成的酶,该酶在糖酵解和糖异生中催化3-磷酸甘油醛和磷酸二羟基丙酮的异构化。该基因的突变与磷酸丙糖异构酶缺乏有关。已在1、4、6和7号染色体上鉴定出假基因。替代拼接导致多个转录本变体。它催化氢原子从碳1转移到碳2,这是一种分子内氧化还原反应。除了在糖酵解中的作用外,它还参与其他几个代谢生物过程,包括糖异生,磷酸戊糖分流和脂肪酸生物合成。TPI1缺乏症是一种疾病,其特征是红细胞缺乏(贫血),运动问题,感染易感性增加以及肌肉无力,可影响呼吸和心脏功能。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠磷酸丙糖异构酶1(TPI1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠磷酸丙糖异构酶1(TPI1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TPI1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TPI1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.