BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经营养因子3(NT-3)是一种蛋白质，由NTF3基因编码。它是神经营养素NGF（Nerve Growth Factor）家族中的一种神经营养因子。它是一种蛋白质生长因子，对周围和中枢神经系统的某些神经元具有活性；它有助于支持现有神经元的生存和分化，并鼓励新神经元和突触的生长和分化。它是继神经生长因子（NGF）和脑源性神经营养因子（BDNF）之后第三个被定性的神经营养因子。鉴于NT-3具有激活两个受体酪氨酸激酶神经营养素受体（TrkC和TrkB）的能力，它能够潜在地刺激的神经元数量是独一无二的。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠神经营养因子3(NT-3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠神经营养因子3(NT-3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse NT-3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse NT-3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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