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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经营养因子4(NT-4),又称神经营养因子5(NT-5),由NTF4基因编码的蛋白质。它是一种神经营养因子,主要通过TrkB受体酪氨酸激酶发出信号。该基因是神经营养因子家族中的一员,即神经营养素,控制着哺乳动物神经元的生存和分化。该基因的表达是无处不在的,受环境信号的影响较小。其他神经营养素的基因敲除,包括神经生长因子、脑源性神经营养因子和神经营养素3,在出生后的早期发育中被证明是致命的,而NTF5缺陷的小鼠只显示轻微的细胞缺陷,并正常发育到成年。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠神经营养因子4(NT-4)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠神经营养因子4(NT-4)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse NT-4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse NT-4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.