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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经调节素是一个由四个结构相关的蛋白组成的家族,属于EGF蛋白家族。这些蛋白已被证明在神经系统的发育中具有不同的功能,并在脊椎动物的胚胎发育中发挥多种重要作用,包括:心脏发育、许旺细胞和少突胶质细胞的分化、神经元发育的某些方面,以及神经肌肉突触的形成。而神经调节蛋白4(NRG4)是神经胶质蛋白家族的成员,由NRG4基因编码。包括NRG4在内的神经球蛋白可激活ERB-B2受体酪氨酸激酶4(ERBB4),通过细胞膜酪氨酸磷酸化启动细胞信号传导。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠神经调节素4(NRG4)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠神经调节素4(NRG4)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse NRG4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse NRG4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.