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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
突触后密度蛋白95(PSD95)也被称为SAP-90(突触相关蛋白90),是一种由DLG4(盘状大同源物4)基因编码的蛋白质。它是膜相关鸟苷酸激酶(MAGUK)家族的一个成员。它与PSD-93一起被招募到相同的NMDA受体和钾通道群中。这两个MAGUK蛋白可能在突触后部位相互作用,形成一个多聚体支架,用于受体、离子通道和相关信号蛋白的聚集。它是PDZ结构域的MAGUK家族中研究得最好的成员。与所有MAGUK家族的蛋白一样,其基本结构包括三个PDZ结构域、一个SH3结构域和一个由无序连接区连接的鸟苷酸激酶样结构域(GK)。它几乎完全位于神经元的突触后密度,并参与锚定突触蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠突触后密度蛋白95(PSD95)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠突触后密度蛋白95(PSD95)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse PSD95. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse PSD95 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.