BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
突触素(SYP)也被称为主要的突触囊泡蛋白p38，是一种在由SYP基因编码的蛋白质。该基因位于X染色体的短臂上（Xp11.23-p11.22）。它的长度为12,406个碱基，位于负链上。编码的蛋白质有313个氨基酸，预测分子量为33.845 kDa。该蛋白是一种突触囊糖蛋白，有四个跨膜结构域，重38kDa。它存在于神经内分泌细胞以及大脑和脊髓中几乎所有参与突触传递的神经元中。它作为神经内分泌肿瘤的标志物，其在突触中的普遍性导致了突触蛋白免疫染色被用于突触的量化。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠突触素(SYP)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠突触素(SYP)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SYP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SYP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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