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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
MYH7是一个编码肌球蛋白重链β(MHC-β)异构体(慢速抽动)的基因,主要在心脏中表达,但也在骨骼肌(I型纤维)中表达。该异构体与心脏肌球蛋白重链的快速异构体MYH6不同,被称为MHC-α。MHC-β是构成心肌厚丝的主要蛋白,在心肌收缩中起主要作用。MHC-β是一个223 kDa的蛋白质,由1935个氨基酸组成。MHC-β是一个六聚体,不对称的马达,形成心肌中厚丝的大部分。MHC-β由N端球状头(20纳米)和α螺旋尾部(130纳米)组成,二聚体和多聚体形成一个螺旋线圈图案,形成轻质子宫肌球蛋白(LMM),厚丝杆。每个MHC-β头的9纳米α螺旋颈部区域非共价地结合两个轻链,基本轻链(MYL3)和调节轻链(MYL2)。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠肌球蛋白重链7(MYH7)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠肌球蛋白重链7(MYH7)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MYH7. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MYH7 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.