计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肝癌衍生生长因子(HDGF),也称为高迁移率族蛋白1样2,是由HDGF基因编码的蛋白质。 它具有有丝分裂和DNA结合活性,可能在细胞增殖和分化中发挥作用。它的高水平表达可以增强许多肿瘤的生长,与它相关的疾病包括肺癌和肝细胞癌。它对成纤维细胞具有促有丝分裂活性,它是肝素结合蛋白。它是一种内源性核靶向有丝分裂原。它是弱保守PWWP域家族的成员。这种70-氨基酸基序最初从WHSC1基因中鉴定出来,已经在60多种真核蛋白中被发现。除了PWWP结构域外,该类中的许多蛋白质还含有已知的染色质重塑结构域,这表明它在染色质重塑中的作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠肝癌衍生生长因子(HDGF)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠肝癌衍生生长因子(HDGF)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse HDGF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse HDGF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.