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肿瘤坏死因子受体 1 型相关死亡域蛋白是一种蛋白质,由 TRADD 基因编码。TRADD 是一种适应性蛋白。该基因编码的蛋白是一种含有死亡域的适应分子,与 TNFRSF1A/TNFR1 相互作用,并介导程序性细胞死亡信号和 NF-κB 的激活。该蛋白与适应蛋白 TRAF2 结合,减少 TRAF2 对凋亡抑制蛋白(IAPs) 的招募,从而抑制 TRAF2 介导的凋亡。该蛋白还能与受体 TNFRSF6/FAS 和适应蛋白 FADD/MORT1 相互作用,并参与 Fas 诱导的细胞死亡途径。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠肿瘤坏死因子受体相关死亡域蛋白(TRADD)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠肿瘤坏死因子受体相关死亡域蛋白(TRADD)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TRADD. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TRADD present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.