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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肿瘤坏死因子受体超家族成员5(TNFRSF5;CD40)是一种在抗原呈递细胞上发现的代价刺激蛋白，是其激活的必要条件。TH细胞上的CD154（CD40L）与CD40的结合能激活抗原呈递细胞，并诱发各种下游效应。该基因所编码的蛋白受体是TNF-受体超家族的成员。该受体已被发现在介导各种免疫和炎症反应中至关重要，包括T细胞依赖的免疫球蛋白类别转换、记忆B细胞发育和生殖中心形成。CD40的表达是多样化的,它由抗原呈递细胞组成性表达，包括树突状细胞、B细胞和巨噬细胞。它也可以由内皮细胞、平滑肌细胞、成纤维细胞和上皮细胞表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠肿瘤坏死因子受体超家族成员5(TNFRSF5;CD40)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠肿瘤坏死因子受体超家族成员5(TNFRSF5;CD40)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TNFRSF5;CD40. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TNFRSF5;CD40 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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