BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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TRAIL是一种作为配体发挥作用的蛋白质，诱导细胞死亡的过程称为凋亡。它也被指定为CD253（分化簇253）和TNFSF10（肿瘤坏死因子（配体）超家族，成员10）。TRAIL与死亡受体DR4（TRAIL-RI）和DR5（TRAIL-RII）结合。细胞凋亡的过程是caspase-8依赖性的。Caspase-8激活下游效应caspase，包括procaspase-3、-6和-7，导致特定激酶的激活。它还结合受体DcR1和DcR2，它们不含细胞质结构域（DcR1）或含有截短的死亡结构域（DcR2）。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠肿瘤坏死因子相关凋亡诱导配体(TRAIL)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠肿瘤坏死因子相关凋亡诱导配体(TRAIL)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TRAIL. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TRAIL present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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