计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胱氨酸/谷氨酸转运蛋白(SLC7A11)是一种抗转运体,由 SLC7A11 基因编码 。SLC7A11 基因编码的是一种依赖钠的胱氨酸-谷氨酸抗转运体,它依赖于氯化物,被称为 Xc-系统或 xCT。它通过刺激突触外受体来调节突触活动, 并进行非静脉谷氨酸的释放。该基因在星形胶质细胞中高度表达,并将一个分子的胱氨酸的吸收与一个分子的谷氨酸的释放联系起来。二聚体半胱氨酸被胶质细胞吸收,单体半胱氨酸被神经元吸收。Xc-的表达在整个大脑中被检测到,在杏仁基底外侧、视网膜和前额叶皮层中表达较高。已经发现对系统Xc-的抑制可以改变一些行为,这表明它在兴奋性信号传递中起着关键作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠胱氨酸/谷氨酸转运蛋白(SLC7A11)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠胱氨酸/谷氨酸转运蛋白(SLC7A11)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SLC7A11. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SLC7A11 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.