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胸腺嘧啶核苷磷酸化酶(TP),也被称为胸苷磷酸化酶或TYMP,是由TYMP基因编码并催化反应的酶。它参与嘌呤代谢、嘧啶代谢和其他代谢途径。胸苷磷酸化酶和编码它的TYMP基因的变异与线粒体神经胃肠性脑病综合征和膀胱癌有关。它是一种具有相同亚基的蛋白质二聚体。它在DNA/RNA降解后回收核苷的嘧啶回收中起关键作用。尽管它在胸苷/脱氧尿苷和它们各自的碱基之间催化的反应是可逆的,但酶的功能主要是分解代谢。它也参与血管生成。它在6-氨基-5-氯尿嘧啶(胸苷磷酸化酶抑制剂 )存在下抑制血管生成作用,这表明它的酶活性是其血管生成活性所必需的。它与血小板衍生的内皮细胞生长因子 (PD-ECGF) 几乎相同。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠胸腺嘧啶核苷磷酸化酶(TP)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠胸腺嘧啶核苷磷酸化酶(TP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.