BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
含脯氨酰羟化酶域的蛋白 2（PHD2），或低氧诱导因子脯氨酰羟化酶 2（HIF-PH2），是 EGLN1 基因编码的一种酶。它也被称为 EGL 九号同源物1。PHD2 是一种α-酮戊二酸/2-氧代戊二酸依赖性羟化酶，属于超家族非含铁蛋白。它是缺氧诱导因子-脯氨酸二氧酶的三种异构体之一，也被称为 HIF 脯氨酸羟化酶。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠脯氨酸羟化酶2(PHD2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠脯氨酸羟化酶2(PHD2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse PHD2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse PHD2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
危险品化学品经营许可证（带存储）