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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
膜联蛋白 A10,也被称为 ANXA10,由 ANXA10 基因编码。该基因编码膜联蛋白家族的一个成员。这种钙依赖性磷脂结合蛋白家族的成员在调节细胞生长和信号转导途径中发挥作用。ANXA10是膜联家族成员,是位于染色体 4q33上的蛋白质编码基因。膜联蛋白包含与膜结合的钙结合蛋白家族。它们具有许多生物活性,包括细胞凋亡、囊泡运输、钙信号传导、生长控制和细胞分裂8。ANXA10 是一种恶性肿瘤抑制剂,敲除 ANXA10 可刺激肺癌细胞侵袭和转移。此外,ANXA10 表达的缺失与早期胃癌的不良预后密切相关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠膜联蛋白A10(ANXA10)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠膜联蛋白A10(ANXA10)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ANXA10. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ANXA10 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.