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苹果酸脱氢酶1，也被称为MDH1，是由MDH1基因编码的酶。苹果酸脱氢酶利用柠檬酸循环中的NAD/NADH辅助因子系统，催化苹果酸盐的可逆氧化成草酰乙酸。该基因编码的蛋白质定位于细胞质上，可能在苹果酸-天冬氨酸穿梭中起关键作用，该穿梭在细胞质基质和线粒体之间的代谢协调中起作用。它的乙酰化激活其酶活性并增强NADPH的细胞内水平，从而促进脂肪生成分化。精氨酸248（R248）的甲基化对MDH1有负面调节。蛋白质精氨酸甲基转移酶4（PRMT4/CARM1）甲基化并通过破坏其二聚化来抑制MDH1。它的精氨酸甲基化抑制线粒体呼吸并抑制谷氨酰胺利用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠苹果酸脱氢酶1(MDH1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠苹果酸脱氢酶1(MDH1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse MDH1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse MDH1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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