计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
葡萄糖调节蛋白-78(Grp78)也称结合免疫球蛋白 (BiPS)或G 蛋白偶联受体 78,是一种蛋白质,是由 GPR78 基因编码的。G 蛋白偶联受体(GPCRs,或 GPRs)含有 7 个跨膜结构域,并通过异源三聚体 G 蛋白转发细胞外信号。Grp78是一种位于内质网 (ER) 腔内的 HSP70 分子伴侣,当新合成的蛋白质转位到 ER 中时,它会结合这些蛋白质,并将它们维持在能够进行后续折叠和寡聚化的状态。Grp78也是易位机制的重要组成部分,在注定要被蛋白酶体降解的异常蛋白穿过 ER 膜的逆行转运中发挥作用。Grp78在所有生长条件下都是一种丰富的蛋白质,但在导致 ER 中未折叠多肽积累的条件下,其合成被显着诱导。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠葡萄糖调节蛋白-78(Grp78)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠葡萄糖调节蛋白-78(Grp78)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Grp78. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Grp78 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.