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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
帕金森氏病蛋白 7(PARK7)又称蛋白脱脂酶 DJ-1,是一种由 PARK7 基因编 码的蛋白质。它由 189 个氨基酸组成,共有 7 个β链和 9 个α螺旋,以二聚 体形式存在。它属于肽酶 C56 家族的蛋白质。在氧化条件下,它通过其伴侣 活性抑制α-突触核蛋白的聚集,从而作为一种对氧化还原敏感的伴侣和氧化 应激的传感器发挥作用。因此,它显然能保护神经元免受氧化应激和细胞死 亡的影响。同时,该蛋白作为雄性激素受体依赖性转录的正向调节剂。它在 哺乳动物的神经视网膜和视网膜色素上皮中都有表达,它在生理和病理条件 下对氧化应激发挥着神经保护作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠蛋白DJ-1(DJ-1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠蛋白DJ-1(DJ-1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse DJ-1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse DJ-1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.