BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血清淀粉样蛋白A3（SAA3）主要来自于肝外组织。SAA3是诱导性SAA蛋白的主要异构体，该蛋白还包括SAA1和SAA2。SAA3的基因缺失表现为促炎症细胞因子的产生增加，IL-22的表达减少，同时上皮细胞破坏加剧，结肠长度减少。与以前报道的通过IL-23诱导IL-22不同，SAA3诱导的中性粒细胞IL-22的表达利用的是TLR2依赖机制，不依赖于IL-23。SAA3在发炎的结肠粘膜中的存在，部分是通过产生IL-22的中性粒细胞的扩张来保护上皮屏障。据推测，SAA3对自体中性粒细胞的刺激可能对炎症性肠病有治疗作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠血清淀粉样蛋白A3(SAA3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血清淀粉样蛋白A3(SAA3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SAA3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SAA3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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