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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血清淀粉样蛋白P(SAP)是淀粉样蛋白P成分(AP)的相同血清形式,是一种25kDa的五聚体蛋白,首次被确认为体内病理沉积物的五边形成分,称为"淀粉样蛋白"。AP占淀粉样蛋白沉积物干重的14%,被认为是一组被称为淀粉样蛋白病的相关疾病的发病机制的重要因素。AP被认为是通过阻止蛋白酶的裂解来装饰和稳定聚集体,从而抑制通过正常的蛋白质清除机制来清除纤维素。SAP-淀粉样蛋白关联也被确定为抗淀粉样蛋白治疗的可能药物靶点。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠血清淀粉样蛋白P(SAP)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血清淀粉样蛋白P(SAP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SAP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SAP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.