BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管假血友病因子（VWF）是一种由血管内皮细胞及巨核细胞合成和分泌的大分子糖蛋白，主要由第12号染色体短臂编码。作为血浆中的关键成分，VWF在止血过程中发挥双重作用：一方面通过与血小板膜糖蛋白GPⅠb-Ⅸ复合物及内皮下胶原结合，介导血小板在血管损伤部位的黏附；另一方面作为凝血因子Ⅷ的载体，保护其稳定性并促进凝血反应。VWF水平异常与多种疾病相关。缺乏VWF会导致血管性血友病，表现为出血倾向；而其水平升高则与血栓性疾病、心肌梗死、糖尿病及妊娠期高血压等病理状态相关。临床常通过检测VWF抗原（VWF Ag）含量评估其功能状态，辅助诊断相关疾病并指导治疗。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠血管假血友病因子抗原(VWF Ag)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血管假血友病因子抗原(VWF Ag)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse VWF Ag. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse VWF Ag present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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