BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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血管生成素样蛋白4（ANGPTL4），是由ANGPTL4基因编码的蛋白质。该基因是血管生成素样基因家族的成员，编码糖基化的分泌蛋白，具有盘绕的N端结构域和纤维蛋白原样C端结构域。它以前称为ANGPTL2，HFARP，PGAR或FIAF，但现已重命名为ANGPTL4。该基因在各种细胞类型的低氧条件下被诱导，并且是过氧化物酶体增殖物激活受体的靶标。编码的蛋白质是直接参与调节脂质代谢的血清激素。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠血管生成素样蛋白4(ANGPTL4)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血管生成素样蛋白4(ANGPTL4)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse ANGPTL4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse ANGPTL4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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