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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管非炎性蛋白 1 也称泛酸酶,是一种酶,是由 VNN1 基因编码的。该基因 的产物是 Vanin 蛋白家族的成员,该家族的蛋白彼此之间有广泛的序列相似 性,也与生物素酶相似。该家族包括分泌蛋白和膜相关蛋白,其中一些蛋白 已被报道参与了造血细胞的贩运。目前还没有发现任何一种万能蛋白具有生 物素酶的活性,但是它们具有泛酸酶的活性,这可能在氧化应激反应中起作 用。该基因与 6q23-q24 号染色体上的另外两个凡尔纳基因相近,且转录方向相同。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠血管非炎性蛋白1(VNN1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血管非炎性蛋白1(VNN1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse VNN1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse VNN1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.