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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血红蛋白(Hemoglobin,Hb、Hgb)又称血红素,俗称血色素,是高等生物体内红色含铁的携氧结合蛋白。血红蛋白的结构为异源四聚体,由2对珠蛋白组成,每个珠蛋白结合1个血红素。哺乳动物的一个血红蛋白分子,可以结合最多四个氧分子。可以用平均细胞血红蛋白浓度测出浓度。人类血红蛋白由2对(4条)血红蛋白单体聚合成四聚体,每个血红蛋白单体由每一条肽链和一个血红素连接构成。肽链有两种(即珠蛋白的两种肽链):含141个氨基酸残基的α链和含146个氨基酸残基的非α链(β、γ及δ链,各种都有固定的氨基酸排列顺序)。其中α链基因位于16号染色体,β、δ、γ链基因位于11号染色体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠血红蛋白(Hb)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠血红蛋白(Hb)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse Hb. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse Hb present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.