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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
表皮生长因子样结构域蛋白7(EGFL7),也被称为含有EGF样结构域的蛋白质7,是由EGFL7基因编码的蛋白质。它的内含子7包含miR-126 microRNA基因。在体外,它抑制PDGF-BB刺激的主动脉平滑肌细胞迁移,但对细胞增殖没有影响,这表明它在血管成熟中起作用。在体内,它敲低表达在肝细胞癌细胞中减少肝内和肺转移的数量。在肿瘤内,它增加微血管密度,缺氧,坏死和血管通透性。它是血管弹性发生的天然负调节剂。它与LOX的催化活性相互作用并抑制,防止对苯弹性蛋白分子交联成成熟的不溶性弹性蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠表皮生长因子样结构域蛋白7(EGFL7)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠表皮生长因子样结构域蛋白7(EGFL7)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse EGFL7. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse EGFL7 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.