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超氧化物歧化酶（SOD，EC 1.15.1.1）是一种交替催化超氧化物（O-2）阴离子自由基歧化（或分离）为正常分子氧（O2）和过氧化氢（H2O2）的酶。超氧化物是氧代谢的副产物，如果不加以调节，会对细胞造成多种损害。因此，在几乎所有暴露于氧气的活细胞中，SOD 都是一种重要的抗氧化防御物质。植物乳杆菌和相关乳酸菌是一个例外，它们利用细胞内的锰来防止活性 O-2 造成的损害。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠超氧化物歧化酶(SOD)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠超氧化物歧化酶(SOD)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse SOD. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse SOD present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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