BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转化受体电位阳离子通道亚家族M成员4（TRPM4），也被称为melastatin-4，是由TRPM4基因编码的蛋白质。它是钙活化的非选择性离子通道，介导一价阳离子在膜上的转运，从而使膜去极化。因此，它在腔肌细胞，内嗅皮层神经元，背根和伏美隆鼻神经元，内分泌胰腺细胞，肾上皮细胞，耳蜗毛细胞等中起核心作用。它通过调节T淋巴细胞活化后的Ca（2+）振荡参与T细胞活化，这是NFAT依赖性IL2产生所必需的。参与脑动脉的肌源性收缩。控制胰腺β细胞中的胰岛素分泌。也可能参与起搏，或在Ca（2+）过载条件下引起不规则的电活动。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠转化受体电位阳离子通道亚家族M成员4(TRPM4)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠转化受体电位阳离子通道亚家族M成员4(TRPM4)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TRPM4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TRPM4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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